Stepan Melnyk, PhD |
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| Research Assistant Professor, Department of Pediatrics, College of Medicine, University of Arkansas for Medical Sciences |
| Phone: (501) 364-4534 |
Research Overview
Dr. Melnyk’s work in the Developmental Pediatric Section is closely related to the research of Dr. Jill James. They have worked more than 10 years together on the field of metabolic genomics and with operating the Metabolic Genomics Laboratory at ACHRI. The laboratory has a continuing, long-time collaboration with the Arkansas Center for Birth Defects Research and Prevention. Dr. Melnyk recently started to organize an analytical core laboratory for Oxidative Stress Biomarkers. His scientific interests include: 1) oxidative stress damage and biomarkers in numerous diseases, including most recently autism, Down syndrome, childhood acute lymphoblastic leukemia, and congenital heard abnormality; 2) the involvement of donors for methylation reaction in biological substrates and DNA methylation quantification in pathogenesis of diseases; and 3) the development of new analytical techniques utilizing MS-LC, HPLC-ECD, HPLC-UV, Corona (universal charged aerosol detector) for future and current research and utilization and adaptation of available techniques for ongoing research.
Key Publications*
Melnyk S, Pogribna M, Pogribny I, Hine RJ, James SJ. A new HPLC method for the simultaneous determination of oxidized and reduced plasma aminothiols using coulometric electrochemical detection. J Nutr Biochem. 1999 Aug;10(8):490-7.
Melnyk S, Pogribna M, Miller BJ, Basnakian AG, Pogribny IP, James SJ. Uracil misincorporation, DNA strand breaks, and gene amplification are associated with tumorigenic cell transformation in folate deficient/repleted Chinese hamster ovary cells. Cancer Lett. 1999 Nov 1;146(1):35-44.
James SJ, Pogribny IP, Pogribna M, Miller BJ, Jernigan S, Melnyk S. Mechanisms of DNA damage, DNA hypomethylation, and tumor progression in the folate/methyl-deficient rat model of hepatocarcinogenesis. J Nutr. 2003 Nov;133(11 Suppl 1):3740S-3747S. Review.
Blossom SJ, Doss JC, Hennings LJ, Jernigan S, Melnyk S, James SJ. Developmental exposure to trichloroethylene promotes CD4+ T cell differentiation and hyperactivity in association with oxidative stress and neurobehavioral deficits in MRL+/+ mice. Toxicol Appl Pharmacol. 2008 Sep 15;231(3):344-53.
S Jill James, Melnyk S, Jernigan S, Hubanks A, Rose S, Gaylor DW. Abnormal Transmethylation/transsulfuration Metabolism and DNA Hypomethylation Among Parents of Children with Autism. J Autism Dev Disord. 2008 Nov;38(10):1966-75.
Rose S, Melnyk S, Savenka A, Hubanks A, Jernigan S, Cleves M, James SJ. The Frequency of Polymorphisms affecting Lead and Mercury Toxicity among Children with Autism. American Journal of Biochemistry and Biotechnology 4 (2): 85-94, 2008.
James SJ, Melnyk S, Fuchs G, Reid T, Jernigan S, Pavliv O, Hubanks A, Gaylor DW. Efficacy of methylcobalamin and folinic acid treatment on glutathione redox status in children with autism. Am J Clin Nutr. 2009 Jan;89(1):425-30.
*To find additional publications by this author, please visit Pubmed Central, a National Institutes of Health-operated site for electronic distribution of life sciences research reports.
Research Support
Arkansas Biosciences Institute
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